Staff

Daisuke AsaiAssociate Professor / Ph.D. (in Science)last update : 2024.08.05

Profile

Name: Daisuke Asai
Affiliation: Laboratory of Microbiology
Subjects: Microbiology
Official title: Associate Professor
Degree: Ph.D. (in Science)

Academic background

Ph.D. Chemistry, Kyushu University, Mar.2003

Career history

Postdoctoral Research Associate, Kyushu University, Apr.2003-Mar.2006
Assistant Professor, Department of Microbiology, St. Marianna University School of Medicine, Apr.2006-Nov.2017
Visiting Scholar, Department of Biomedical Engineering, Duke University, Aug.2009-Jul.2011
Junior Associate Professor, Department of Microbiology, St. Marianna University School of Medicine, Dec.2017-Aug.2020
Associate Professor, Laboratory of Microbiology, Showa Pharmaceutical University, Sep.2020-present

Books

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Screening, stabilization and formulation technology of peptide drugs 　Asai D, Nakashima H.　Depot formulation for anti-HIV peptides (in Japanese)　248-257　Technical Information Institute CO., LTD　2017　978-4-86104-687-2 C3047

Papers

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[Original paper]　Activated tyrosine kinase c-Src-responsive artificial gene carrier: in vitro and in vivo evaluation　Terada K, Asai D, Kang J.H., Mori T, Katayama Y.　ChemistrySelect　9　e202303331　2024　
[Review]　Bisphenol A (BPA) and cardiovascular or cardiometabolic diseases　Kang JH, Asai D, Toita R.　J. Xenobiot.　13　775-810　2023　
[Original paper]　Synthesis, odour characteristics, and antimicrobial activity of optically active (Z)-7-decen-4-olide and its derivatives　Shimotori Y, Ogawa N, Mamada S, Miyakoshi T, Masuda H, Kitago S, Shima T, Asai D, Kanamoto T.　J. Oleo Sci.　72　1037-1048　2023　
[Original paper]　Synthesis and cytotoxic mechanism of sulfated alkyl oligosaccharides having potent anti-HIV activity　Mingxue B, Asai D, Koshikawa T, Takemura H, Miyazaki K, Yoshida T.　J. Fiber Sci. Technol.　79　101-111　2023　
[Original paper]　Electrostatic interaction between sulfated polysaccharides and oligopeptides from viral envelope glycoproteins using surface plasmon resonance　Battulga T, Dagaerbieke A, Bai C, Asai D, Koshikawa T, Takemura H, Miyazaki K, Yoshida T.　Carbohydr. Res.　528　108815　2023　
[Original paper]　Importance of accessibility to the extracellular juxtamembrane stalk region of membrane protein for substrate recognition by viral ubiquitin ligase K5　Kajikawa M, Hata H, Ishimura M, Imaizumi N, Kimura M, Miyano K, Ose T, Asai D, Ishido S, Kanamoto T.　Biochem J　479(20)　2261-2278　2022　
[Original paper]　A model of transcriptional activation of DNA by loosening of chromatin structure　Sato YT, Asai D, Terada K, Kang JH, Mori T, Niidome T, Nakashima H, Katayama Y　Chem Lett　51(12)　1109-1112　2022　
[Original paper]　Old but still useful [γ-32P]ATP ―Development of peptide substrates for protein kinases by 32P-based enzyme activity assay―　Asai D, Kang JH, Katayama Y　BUNSEKI KAGAKU　71(3)　179-185　2022　
[Review]　Rapid virus inactivation by lignosulfonate　Sakagami H, Fukuchi K, Asai D, Kanamoto T, Koshikawa T, Takemura H, Inomata M, Aimi H, Kikkawa Y　New Food Industry　64(2)　106-110　2022　
[Original paper]　Direct evidence of edge-to-face CH/π interaction for PAR-1 thrombin receptor activation　Asai D, Inoue N, Sugiyama M, Fujita T, Matsuyama Y, Liu X, Matsushima A, Nose T, Costa T, Shimohigashi Y　Bioorg Med Chem　51　116498-116498　2021　
[Original paper]　Lignosulfonate rapidly inactivates human immunodeficiency and herpes simplex viruses　Fukuchi K, Koshikawa T, Asai D, Inomata M, Sakagami H, Takemura H, Kanamoto T, Aimi H, Kikkawa Y　Medicines (Basel)　8(10)　e56　2021　
[Original paper]　Evaluation of a new point-of-care quantitative reverse transcription polymerase chain test for detecting severe acute respiratory syndrome coronavirus 2　Watanabe Y, Oikawa R, Suzuki T, Funabashi H, Asai D, Hatori Y, Takemura H, Yamamoto H, Itoh F　J Clin Lab Anal　35　e23992　2021　
[Original paper]　Re-evaluation of chemotherapeutic potential of pyoktanin blue　Sakagami H, Furukawa T, Satoh K, Amano S, Iijima Y, Koshikawa T, Asai D, Fukuchi K, Takemura H, Kanamoto T, Yokose S　Medicines (Basel)　8(7)　e33　2021　
[Review]　Rapid virus inactivation by alkaline extract of leaves Sasa sp.　Sakagami H, Fukuchi K, Asai D, Terakubo S, Takemura H, Horiuchi M, Fujisawa T, Suguro M, Toeda K, Yasui T, Oizumi H, Oizumi T.　New Food Industry　62(11)　785-790　2020　
[Original paper]　Quantification of the ability of natural products to prevent herpes virus infection　Fukuchi K, Sakagami H, Sugita Y, Takao K, Asai D, Terakubo S, Takemura H, Ohno H, Horiuchi M, Suguro M, Fujisawa T, Toeda K, Oizumi H, Yasui T, Oizumi T.　Medicines(Basel)　7　e64　2020　
[Original paper]　Quantification of antitumor, antiviral and neuroprotective activity of twenty Kampo preparations　Shi H, Fukuchi K, Asai D, Terakubo S, Takemura H, Sakagami H.　New Food Industry　62(8)　599-607　2020　
[Review]　Design of substrates and inhibitors of G protein-coupled receptor kinase 2 (GRK2) based on its phosphorylation reaction　Kang JH, Toita R, Kawano T, Murata M, Asai D.　Amino Acids　52(6-7)　863-870　2020　
[Original paper]　Blood retention and antigenicity of polycarboxybetaine-modified liposomes　Ryujin T, Shimizu T, Miyahara R, Asai D, Shimazui R, Yoshikawa T, Kishimura A, Mori T, Ishida T, Katayama Y.　Int J Pharm　586　119521　2020　
[Original paper]　Elucidation of anti-HIV mechanism of sulfated cellobiose–polylysine dendrimers　Weiyue S, Ying L, Kanamoto T, Asai D, Takemura H, Nakashima H, Miyazaki K, Yoshida T.　Carbohydr Res　495　108084　2020　
[Original paper]　Role of a long-chain alkyl group in sulfated alkyl oligosaccharides with high anti-HIV activity revealed by SPR and DLS　Mingxue B, Chaolumen B, Asai D, Takemura H, Miyazaki K, Yoshida T.　Carbohydr Polym　245　116518　2020　
[Original paper]　Effect of fetal bovine serum concentration on lysophosphatidylcholine-mediated proliferation and apoptosis of human aortic smooth muscle cells　Asai D, Kawano T, Murata M, Nakashima H, Toita R, Kang JH.　J Oleo Sci　69(3)　255-260　2020　
[Original paper]　Synthesis and anti-HIV activity of sulfated oligosaccharide-branched β-CD　Mingxue B, Chaolumen B, Asai D, Miyazaki K, Yoshida T. 　J Fiber Sci Technol　76(1)　63-71　2020　
[Original paper]　Injectable polypeptide hydrogel depot system for assessment of the immune-response-inducing efficacy of sustained antigen release alone　Asai D, Fukuda T, Morokuma K, Funamoto D, Yamaguchi Y, Mori T, Katayama Y, Shibayama K, Nakashima H.　Macromol Biosci　19(10)　e1900167　2019　
[Original paper]　Suppression of lysophosphatidylcholine-induced human aortic smooth muscle cell calcification by protein kinase A inhibition　Toita R, Asai D, Otani K, Kawano T, Murata M, Kang JH.　Lipids　54(8)　465-470　2019　
[Original paper]　A high?affinity peptide substrate for G protein?coupled receptor kinase 2 (GRK2)　Asai D, Murata M, Toita R, Kawano T, Nakashima H, Kang JH　Amino Acids　51(6)　973-976　2019　
[Review]　Pathogenic viruses commonly present in the oral cavity and relevant antiviral compounds derived from natural products　Asai D, Nakashima H.　Medicines(Basel)　5(4)　e120　2019　
[Original paper]　A peptide inhibitor of antibody-dependent cell-mediated cytotoxicity against EGFR/folate receptor-α double positive cells　Sasaki K, Miyashita Y, Asai D, Funamoto D, Sato K, Yamaguchi Y, Mishima Y, Iino T, Takaishi S, Nagano J, Kishimura A, Mori T, Katayama Y.　MedChemComm　9(5)　783-788　2018　
[Original paper]　In situ depot formation of anti-HIV fusion-inhibitor peptide in recombinant protein polymer hydrogel　Asai D, Kanamoto T, Takenaga M, Nakashima H.　Acta Biomater　64　116-125　2017　
[Original paper]　Role of amino acid residues surrounding the phosphorylation site in peptide substrates of G protein-coupled receptor kinase 2 (GRK2)　Asai D, Murata M, Toita R, Kawano T, Nakashima H, Kang JH.　Amino Acids　48(12)　2875-2880　2016　
[Original paper]　Reversal of efflux of an anticancer drug in human drug-resistant breast cancer cells by inhibition of protein kinase Cα (PKCα) activity　Kim CW, Asai D, Kang JH, Kishimura A, Mori T, Katayama Y.　Tumour Biol　37(2)　1901-1908　2016　
[Original paper]　Spatiotemporally photoradiation-controlled intratumoral depot for combination of brachytherapy and photodynamic therapy for solid tumor　Mukerji R, Schaal J, Li X, Bhattacharyya J, Asai D, Zalutsky MR, Chilkoti A, Liu W.　Biomaterials　79　79-87　2016　
[Original paper]　Expression and characterization of myristoylated preS1-conjugated nanocages for targeted cell delivery　Murata M, Piao JS, Narahara S, Kawano T, Hamano N, Kang JH, Asai D, Ugawa R, Hashizume M.　Protein Expr Purif　110　52-56　2015　
[Original paper]　Monitoring of phosphorylated peptides by radioactive assay and matrix-assisted laser desorption-ionization time-of-flight mass spectrometry　Kang JH, Asai D, Toita R, Kawano T, Murata M.　Amino Acids　47(11)　2377-2383　2015　
[Original paper]　Potential new chemotherapy strategy for human ovarian carcinoma with a novel KSP inhibitor　Takenaga M, Yamamoto Y, Takeuchi T, Ohta Y, Tokura Y, Hamaguchi A, Asai D, Nakashima H, Oishi S, Fujii N.　Biochem Biophys Res Commun　463(3)　222-228　2015　
[Original paper]　Tandemly repeated peptide for cancer-specific gene carrier prepared by native chemical ligation 　Funamoto D, Asai D, Kim CW, Nakamura Y, Lee EK, Nobori T, Niidome T, Mori T, Katayama Y　Chem Lett　44(4)　474-476　2015　
[Original paper]　Antibody internalization into living cell via crosslinker-mediated endocytosis　Funamoto D, Asai D, Sato K, Yamaguchi Y, Kim CW, Sato H, Nakhaei E, Matsumoto S, Yoshikawa T, Sasaki K, Yamamoto T, Kishimura A, Mori T, Katayama Y.　Chem Lett　44(4)　468-470　2015　
[Original paper]　Reduction of inorganic phosphate-induced human smooth muscle cells calcification by inhibition of protein kinase A and p38 mitogen-activated protein kinase　Kang JH, Toita R, Asai D, Yamaoka T, Murata M.　Heart Vessels　29(5)　718-722　2014　
[Original paper]　Liver cell-specific peptides derived from the PreS1 domain of human hepatitis B virus　Kang JH, Toita R, Asai D, Yamaoka T, Murata M.　J Virol Methods　201　20-23　2014　
[Original paper]　Peptide substrates for G protein-coupled receptor kinase 2　Asai D, Toita R, Murata M, Katayama Y, Nakashima H, Kang JH.　FEBS Lett　588(13)　2129-2132　2014　
[Original paper]　Brachytherapy using injectable seeds that are self-assembled from genetically encoded polypeptide in situ　Liu W, McDaniel JR, Li X, Asai D, Garcia Quiroz F, Schaal J, Park JS, Zalutsky MR, Chilkoti A.　Cancer Res　72(22)　5956-5965　2012　
[Original paper]　Rheological properties of cysteine-containing elastin-like polypeptides solution and hydrogels　Xu D, Asai D, Chilkoti A, Craig SL.　Biomacromolecules　13(8)　2315-2321　2012　
[Original paper]　Protein polymer hydrogels by in situ, rapid and reversible self-gelation　Asai D, Xu D, Liu W, Garcia Quiroz F, Callahan DJ, Zalutsky MR, Craig SL, Chilkoti A.　Biomaterials　33(21)　5451-5458　2012　
[Original paper]　Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and radioactivities by radioactive assay　Tsuchiya A, Asai D, Kang JH, Mori T, Niidome T, Katayama Y.　Anal Biochem　421(2)　773-775　2012　
[Original paper]　Transgene regulation system responding to Rho associated coiled-coil kinase (ROCK) activation　Tsuchiya A, Kang JH, Asai D, Mori T, Niidome T, Katayama Y.　J Control Release　155(1)　40-46　2011　
[Original paper]　Peptide substrates for Rho-associated kinase 2 (Rho-kinase 2/ROCK2)　Kang JH, Asai D, Tsuchiya A, Mori T, Niidome T, Katayama Y.　PLoS One　6(7)　e22699　2011　
[Original paper]　Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule　Kang JH, Oishi J, Kim JH, Ijuin M, Toita R, Jun B, Asai D, Mori T, Niidome T, Tanizawa K, Kuroda S, Katayama Y.　Nanomedicine　6(4)　583-589　2010　
[Original paper]　Tumor therapy by gene regulation system responding to cellular signal　Tomiyama T, Toita R, Kang JH, Asai D, Shiosaki S, Mori T, Niidome T, Katayama Y.　J Control Release　148(1)　101-105　2010　
[Review]　D-RECS: Development of disordered cell-specific gene regulation system (appeared in "Young square"(mini review)) (in Japanese)　Asai D.　Drug Delivery System　25(4)　426-427　2010　
[Review]　Progress in current non-viral carriers for gene delivery　Shoji-Moskowitz Y, Asai D, Kodama K, Katayama Y, Nakashima H.　Front Med Chem　5(1)　81-97　2010　
[Original paper]　Specific transgene expression in HIV-infected cells using protease-cleavable transcription regulator 　Asai D, Kuramoto M, Shoji Y, Kang JH, Kodama KB, Kawamura K, Mori T, Miyoshi H, Niidome T, Nakashima H, Katayama Y.　J Control Release　141(1)　52-61　2010　
[Original paper]　Molecular mechanism of caspase-3-induced gene expression of polyplexes formed from polycations grafted with cationic substrate peptides　Kawamura K, Kuramoto M, Mori T, Toita R, Oishi J, Sato Y, Kang JH, Asai D, Niidome T, Katayama Y.　J Biomater Sci Polym Ed　20(7-8)　967-980　2009　
[Original paper]　Cellular signal-specific peptide substrate is essential for the gene delivery system responding to cellular signals　Kang JH, Toita R, Tomiyama T, Oishi J, Asai D, Mori T, Niidome T, Katayama Y.　Bioorg Med Chem Lett　19(21)　6082-6086　2009　
[Original paper]　Plasma protein kinase C (PKC)alpha as a biomarker for the diagnosis of cancers　Kang JH, Asai D, Toita R, Kitazaki H, Katayama Y.　Carcinogenesis　30(11)　1927-1931　2009　
[Original paper]　Regulation of transgene expression in tumor cells by exploiting endogenous intracellular signals 　Asai D, Kang JH, Toita R, Tsuchiya A, Niidome T, Nakashima H, Katayama Y.　Nanoscale Res Lett　4(3)　229-233　2009　
[Original paper]　Inflammatory cell-specific transgene expression system responding to Ikappa-beta kinase beta activation　Asai D, Tsuchiya A, Kang JH, Kawamura K, Oishi J, Mori T, Niidome T, Shoji Y, Nakashima H, Katayama Y.　J Gene Med　11(7)　624-632　2009　
[Original paper]　Correlation between phosphorylation ratios by matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis and enzyme kinetics　Kang JH, Kuramoto M, Tsuchiya A, Toita R, Asai D, Sato YT, Mori T, Niidome T, Katayama Y.　Eur J Mass Spectrom　14(4)　261-265　2008　
[Original paper]　Role of plasma as activator and cofactor in phosphorylation catalyzed by protein kinase C　Kang JH, Asai D, Oishi J, Kawamura K, Toita R, Jiang Y, Mori T, Niidome T, Katayama Y.　Cell Biochem Funct　26(1)　70-75　2008　
[Original paper]　Disordered cell-specific gene regulation system for suicide gene therapy　Asai D, Nakashima H.　Jpn J Chemother　56　538-542　2008　
[Original paper]　Drug delivery system based on responses to an HIV infectious signal　Asai D, Kodama KB, Shoji Y, Nakashima H, Kawamura K, Oishi J, Kuramoto M, Niidome T, Katayama Y.　Med Chem　4(4)　386-391　2008　
[Original paper]　A short peptide is a protein kinase C (PKC) alpha-specific substrate　Kang JH, Asai D, Yamada S, Toita R, Oishi J, Mori T, Niidome T, Katayama Y.　Proteomics　8(10)　2006-2011　2008　
[Original paper]　Design of polymeric carriers for cancer-specific gene targeting: utilization of abnormal protein kinase Calpha activation in cancer cells　Kang JH, Asai D, Kim JH, Mori T, Toita R, Tomiyama T, Asami Y, Oishi J, Sato YT, Niidome T, Jun B, Nakashima H, Katayama Y.　J Am Chem Soc　130(45)　14906-14907　2008　
[Original paper]　Direct measure of fluorescence intensity for efficient receptor-binding assay: Conjugates of ethinylcarboxyestradiol and 5(and 6)-carboxyfluorescein via α,ω-diaminoalkanes as a tracer for etrogen receptor　Asai D, Tokunaga T, Kondo K, Kawaguchi T, Takayanagi S, Shinmyozu T, Nakai M, Yakabe Y, Shimohigashi Y.　J Biochem　143(6)　781-792　2008　
[Review]　Artificial regulation of transgene expression using specific substrate peptides (in Japanese)　Asai D.　Peptide Newsletter Japan　67　8-11　2008　
[Original paper]　Direct, real-time, simultaneous monitoring of intravitreal nitric oxide and oxygen in endotoxin-induced uveitis in rabbits　Yomura Y, Shoji Y, Asai D, Murakami E, Ueno S, Nakashima H.　Life Sci　80(16)　1449-1457　2007　
[Review]　Bisphenol A in the aquatic environment and its endocrine-disruptive effects on aquatic organisms　Kang JH, Asai D, Katayama Y.　Crit Rev Toxicol　37(7)　607-625　2007　
[Review]　Drug delivery system responding to cellular signals in infected cells (in Japanese)　Shoji Y, Kodama K, Asai D, Katayama Y, Nakashima H.　Drug Delivery System　21(5)　505-515　2006　
[Original paper]　Molecular cloning and circadian expression profile of insect neuropeptide PDF in black blowfly, Phormia regina　Matsushima A, Yokotani S, Liu X, Sumida K, Honda T, Sato S, Kaneki A, Takeda Y, Chuman Y, Ozaki M, Asai D, Nose T, Onoue H, Ito Y, Tominaga Y, Shimohigashi Y, Shimohigashi M.　Lett Pept Sci　10(5-6)　419-430　2003　
[Original paper]　Synthesis of a complete set of L-difluorophenylalanines, L-(F-2)Phe, as molecular explorers for the CH/-pi interaction between peptide ligand and receptor　Fujita T, Nose T, Matsushima A, Okada K, Asai D, Yamauchi Y, Shirasu N, Honda T, Shigehiro D, Shimohigashi Y.　Tetrahedron Lett　41(6)　923-927　2000　
[Review]　The assessment of xenoestrogens by competitive receptor binding assays (in Japanese)　Asai D, Shimohigashi Y.　Nihon Rinsho　58　112-116　2000　
[Original paper]　Structural essentials of xenoestrogen dialkyl phthalates to bind to the estrogen receptors　Asai D, Tahara Y, Nakai M, Yakabe Y, Takatsuki M, Nose T, Shinmyozu T, Shimohigashi Y.　Toxicol Lett　118(1-2)　1-8　2000　
[Original paper]　Structural requirements of para-alkylphenols to bind to estrogen receptor　Tabira Y, Nakai M, Asai D, Yakabe Y, Tahara Y, Shinmyozu T, Noguchi M, Takatsuki M, Shimohigashi Y.　Eur J Biochem　262(1)　240-245　1999　
[Original paper]　Binding characteristics of dialkyl phthalates for the estrogen receptor　Nakai M, Tabira Y, Asai D, Yakabe Y, Shimyozu T, Noguchi M, Takatsuki M, Shimohigashi Y.　Biochem Biophys Res Commun　254(2)　311-314　1999

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Conference, etc.

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[Poster presentation]　Design and preparation of infection-sensing peptide depot for Staphylococcus aureus　Hayashi, M, Kawamura, K,